Partial purification of extracellular exo-inulinase from Ulocladium atrum
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چکیده
منابع مشابه
Purification, characterization, gene cloning and preliminary X-ray data of the exo-inulinase from Aspergillus awamori.
Extracellular exo-inulinase has been isolated from a solid-phase culture of the filamentous fungus Aspergillus awamori var. 2250. The apparent molecular mass of the monomer enzyme was 69 +/- kDa, with a pI of 4.4 and a pH optimum of 4.5. The enzyme hydrolysed the beta-(2-->1)-fructan (inulin) and beta-(2-->6)-fructan (levan) via exo-cleavage, releasing fructose. The values for the Michaelis con...
متن کاملProduction and properties of a bacterial thermostable exo-inulinase.
Inulinase and Invertase Activities, Thermophilic Bacilli, Enzyme Thermostability Enzyme production of newly isolated thermophilic inulin-degrading Bacillus sp. 11 strain was studied by batch cultivation in a fermentor. The achieved inulinase and invertase activities after a short growth time (4.25 h) were similar or higher compared to those reported for other mesophilic aerobic or anaerobic the...
متن کاملShort Communication Degradative Oxidation Products of the Melanin of Ulocladium Atrum
It is known that many soil fungí synthesize brown, large aromatic polymers (fungal melanins), chemically related to soil humic acids (Martin and Haider, 1971; Schnitzer and Neyroud, 1975). Since the chemical constituents (or at least, their degradation products) and the physicochemical properties of fungal melanins are comparable to those of humic acids, it is possible that fungal metabolism ma...
متن کاملInteraction of Ulocladium atrum, a Potential Biological Control Agent, with Botrytis cinerea and Grapevine Plantlets
The effectiveness of biological control agent, Ulocladium atrum (isolates U13 and U16) in protecting Vitis vinifera L. cv. Chardonnay against gray mold disease caused by Botrytis cinerea, and simulation of the foliar defense responses was investigated. A degraded mycelium structure during cultural assay on potato dextrose agar revealed that U. atrum isolates U13 and U16 were both antagonistic t...
متن کاملPartial Purification of the Extracellular Hemolysin of Pseudomonas Aeruginosa.
Berk, Richard S. (Wayne State University, College of Medicine, Detroit, Mich.). Partial purification of the extracellular hemolysin of Pseudomonas aeruginosa. J. Bacteriol. 88:559-565. 1964.-Through a series of chemical fractionation steps, the extracellular hemolysin of Pseudomonas aeruginosa was purified 126-fold with a recovery of 49%. Hemolytic activity of crude preparations was irreversibl...
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ژورنال
عنوان ژورنال: Journal of Genetic Engineering and Biotechnology
سال: 2014
ISSN: 1687-157X
DOI: 10.1016/j.jgeb.2014.04.001